The most common method of fecal sampling is with a universal tube that contains a small spoon on its lid. Calprotectin is then assessed by enzyme-linked immunosorbent assay (ELISA) in an extract made from a stool sample of less than 1 g. It is important to note that there are numerous manufacturers of ELISA kits, and all use different antibodies and extraction mediums, which results in different normal ranges and other differences that may involve the sensitivity of the test.
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